Composite

Part:BBa_J85902:Design

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)


Hef1a-LacO1Oid-B1-EYFP-FF4x4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal NheI site found at 307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal BglII site found at 685
    Illegal BamHI site found at 270
    Illegal BamHI site found at 1354
    Illegal XhoI site found at 1084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
    Illegal NgoMIV site found at 819
    Illegal AgeI site found at 95
    Illegal AgeI site found at 1360
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

see basic parts


Source

various

References